Journal: PLoS ONE

Article Title: DIOL Triterpenes Block Profibrotic Effects of Angiotensin II and Protect from Cardiac Hypertrophy

doi: 10.1371/journal.pone.0041545

Figure Lengend Snippet: Time course of angiotensin II (Ang II; 1 µM)-stimulated collagen I protein production (A). Effect of erythrodiol (ERY; 5 µM) or uvaol (UVA; 5 µM) on CTGF (B), collagen I (C) and galectin 3 (D) protein expression in angiotensin II-treated cardiac myofibroblasts for 12 hours. Representative immunoblots of 4 experiments. Values are mean ± SEM of four assays. *p<0.05 vs vehicle. †p<0.05 vs angiotensin II. Quantification of band intensities was measured by densitometry and normalized to respective α-tubulin.

Article Snippet: Membranes were probed with primary antibody for α-SMA (Biocare Medical, CA, USA), PPAR-γ (Santa Cruz, Inc, USA), collagen I (AbD Serotec, Oxford, UK), connective tissue growth factor (CTGF; Torrey Pines Biolabs Inc, East Orange, NJ) and galectin 3 (Thermo Scientific, Rockford, IL) followed by incubation with an HRP-linked secondary antibody.

Techniques: Expressing, Western Blot